Timing of Early Developmental Stages in Embyos of Tripneustes gratilla (Linnaeus, 1758) (Echinodermata: Echinoidea)
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Sea urchin is one of the most important subjects in developmental biology studies due to its rapid and simple development. Timing for its development is a key component for most experiments. However, available data often vary due to the effect of temperature and other external factors. With this, standardization on the timing of the early developmental stages in embryos of Tripneustes gratilla was conducted in this study. Induced spawning and in-vitro fertilization were done on the collected sea urchins. Morphology of the embryo and timing for each developmental stage including early cleavage stages, morula, and blastula were studied. Sea urchin embryos started its development at 2 minutes after fertilization and reached blastula stage after 6 hours. Developmental stages of T. gratilla embryos exhibited embryological distinction from one another and tend to develop rapidly after fertilization making it an appropriate model organism for biological researches.
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